Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Lactate-driven lactylation of HNRNPA1 orchestrates PKM2 splicing and glycolytic reprogramming in bladder cancer

doi: 10.1186/s13046-025-03591-5

Figure Lengend Snippet: Polydatin inhibits HNRNPA1 lactylation and its biological activity in bladder cancer. A Binding free energy of ten candidate compounds docked to the HNRNPA1 active pocket. B Chemical structure of polydatin. C Docking visualization of polydatin within the active pocket of HNRNPA1. D IC 50 values of polydatin in the EJ-1 cell line, as determined by CCK8 assay. E EJ-1 cells were lysed and immunoprecipitated using an anti-HNRNPA1 antibody, followed by detection of HNRNPA1-K350 lactyl lysine. F Colony formation assays were performed to assess the proliferative capacity of EJ-1 cells following 24-h treatment with 30 μM polydatin or 20 mM NaLa. G-H Tumor gross images and tumor weights were assessed in nude mice after subcutaneous injection of EJ-1 cells. Tumors were harvested on Day 19 after subcutaneous implantation. Statistical analyses were performed with unpaired two-tailed Student’s t-test ( n = 5). I Tumor volume measurements throughout the experiment. J Immunohistochemical staining visualized PKM2 levels in xenograft tumors. Scale bars: 100 μm (left panel), 50 μm (right panel). K Schematic representation of the proposed mechanism of HNRNPA1 lactylation. * p < 0.05, ** p < 0.01

Article Snippet: Cells were treated with polydatin (27208–80-6, TargetMol, USA) for 24 h to examine cell proliferation.

Techniques: Activity Assay, Binding Assay, CCK-8 Assay, Immunoprecipitation, Injection, Two Tailed Test, Immunohistochemical staining, Staining

Journal: MethodsX

Article Title: An accessible HPLC-DAD method for the direct detection of acrolein-trapping compounds in complex plant matrices ☆

doi: 10.1016/j.mex.2025.103747

Figure Lengend Snippet: Chromatographic profile of the ten-component mixture, with detection at a wavelength of 280 nm. The upper chromatogram corresponds to the negative control (0 mM acrolein), while the lower chromatogram represents the mixture following pretreatment with 5 mM acrolein. 1: protocatechuic acid, 2: esculin, 3: catechin, 4: epicatechin, 5: coumaric acid, 6: piceid, 7: rutin, 8: phloridzin, 9: resveratrol, 10: phloretin. Acrolein-trapping compounds are highlighted in red.

Article Snippet: 2,4-Dinitrophenylhydrazine hydrochloride (DNPH), aminoguanidine hydrochloride, esculin, piceid and phloretin were obtained from TCI Chemicals.

Techniques: Negative Control